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Invitrogen™ β-Gal Staining Kit

Catalog No. K146501
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K146501 1 Kit
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Catalog No. K146501 Supplier Invitrogen™ Supplier No. K146501
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Description

Includes

Sufficient reagents to stain fifty 60mM plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10μg of pcDNA 3.1/His/lacZ control vector.

Determine percentage of transfected cells expressing lacZ

  • lacZ is bacterial gene often used as reporter construct in eukaryotic transfection experiments
  • Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
  • β-galactosidase catalyzes hydrolysis of X-gal, producing a blue color that can be easily visualized under microscope
  • Colorimetric detection

Beta-Galactosidase Assays/Enzyme and Protein Activity Assays/Proteins, Expression, Isolation and Analysis/Reporter Assays

Specifications

Content And Storage The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 μg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Assay β-Gal (lacZ) Assay
Detection Method Colorimetric
Product Type Beta-Gal Staining Kit
Substrate X-Gal
Quantity 1 Kit
Target Beta-Galactosidase

For Research Use Only. Not for use in diagnostic procedures.