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Invitrogen™ FreeStyle™ MAX CHO Expression System

Catalog No. K900020
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Catalog No. K900020 Supplier Invitrogen™ Supplier No. K900020
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Description

Rapid and high-yield mammalian protein production

FreeStyle MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle cells with high efficiency. The FreeStyle MAX Expression System combines GIBCO™ FreeStyle Media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

  • Easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
  • Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
  • Simplified downstream purification of secreted proteins with FreeStyle serum-free medium
  • Straightforward protocol for production scale-up
  • Replace time-consuming stable cell line generation with large-scale functional protein production in one week

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection, Transient Protein Production and High-Throughput Screening

Specifications

Cell Line FreeStyle™ CHO-S Cells
Classification Animal Origin-Free, Serum-Free
Content And Storage Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information.
Delivery Type Transfection
Culture Type Suspension Cell Culture
Cell Type CHO
Transfection Technique Lipid-based Transfection
Expression Mechanism Cell-Based Expression
Expression System Mammalian
For Use With (Application) Protein Expression
Key Functions Protein Production
Product Line Freestyle
Product Type Expression System
Quantity 1 kit
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Frequently Asked Questions (FAQs)

I am interested in a mammalian expression system that will allow me to obtain very high yields of my protein. What kind of systems do you offer?

For stable high-yield expression, we offer the Freedom CHO-S and Freedom DG44 kits. For transient high-yield expression, we offer the ExpiCHO Expression System, Expi293 Expression System, FreeStyle 293 Expression System, FreeStyle Max 293 Expression System, and FreeStyle Max CHO Expression System. For high-yield expression of functional membrane proteins in Exp293F cells, we offer the Expi293 MembranePro Expression System (Cat. Nos. A25869, A25870) that combines the scalability and ease of use of Expi293 and the technology of MembranePro to allow an increase of more than 20-fold in membrane protein yield compared to the standard, adherent culture MembranePro Functional Protein Expression System. Please see the Application Note (http://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/ProteinExpressionAnalysis/pdfs/PG1391-PJ6257-CO28704-Expi293Adaption-MembraneProTech_AppNoteGlobal-FHR.pdf) for more details.
What vector do you recommend using with FreeSytle MAX CHO Expression System (Cat. No. K900020)?

We recommend using the pcDNA 3.4 TOPO TA Cloning Kit (Cat. No. A14697).

For Research Use Only. Not for use in diagnostic procedures.