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Invitrogen™ FreeStyle™ MAX 293 Expression System
Description
FreeStyle MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle cells with high efficiency. The FreeStyle MAX Expression System combines GIBCO™ FreeStyle Media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture
FreeStyle MAX System provides:
- Easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
- Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
- Simplified downstream purification of secreted proteins with FreeStyle serum-free medium
- Straightforward protocol for production scale-up
- Replace time-consuming stable cell line generation with large-scale functional protein production in one week
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection, Transient Protein Production and High-Throughput Screening
Specifications
Specifications
| Cell Line | FreeStyle™ 293-F Cells |
| Classification | Animal Origin-Free, Serum-Free |
| Content And Storage | Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information. |
| Delivery Type | Transfection |
| Culture Type | Suspension Cell Culture |
| Cell Type | 293-derived Cells |
| Transfection Technique | Lipid-based Transfection, Transient Transfection |
| Expression Mechanism | Cell-Based Expression |
| Expression System | Mammalian |
| For Use With (Application) | Protein Expression |
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Frequently Asked Questions (FAQs)
The two systems have the same components except for the transfection reagent and the medium supplied for formation of transfection complexes. The FreeStyle Max 293 Expression System contains FreeStyle Max Reagent and OptiPRO SFM (serum-free medium that is devoid of animal-origin components), whereas the FreeStyle 293 Expression System contains 293fectin Transfection Reagent and Opti-MEM I Reduced Serum Medium (serum-free medium but not animal-origin free).
The optimal amount will vary, but we recommend starting with a 1:1 ratio of DNA and lipid. For example, 1.25 ug DNA and 1.25 ul of the reagent for one ml of suspension cells.
Generally, any mammalian expression vector should be compatible with the FreeStyle expression system. Some of the more popular choices include pcDNA3.3-TOPO TA vector (Cat. No. K8300-01), pcDNA3.4-TOPO TA vector (Cat. no. A14697), pcDNA3.2 DEST vector (Cat. No. 12489018), pCEP vector (Cat. No. V044-50), or pcDNA4/HisMax vector (Cat. No. K864-20).
Lipofectamine 2000 is designed for adherent cells. FreeStyle and 293fectin reagents are designed for suspended Hek293 and CHO cells. For transfecting 293 suspension cells, FreeStyle MAX reagent yields transfection efficiency comparable to Lipofectamine 2000 and 293fectin reagents. However, Lipofectamine 2000 and 293fectin reagents do not work well for CHO suspension cells.
In general, the transfection efficiency for FreeStyle MAX reagent is 65% when used with suspension CHO and 293 cells.
For Research Use Only. Not for use in diagnostic procedures.