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Invitrogen™ FreeStyle™ 293-F Cells
Description
Animal origin-free, chemically defined, low-protein, reduced growth factors, reduced serum, xeno-free. The FreeStyle 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle MAX 293 Expression System or FreeStyle 293 Expression System. FreeStyle 293-F cells are adapted to suspension culture in FreeStyle 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle 293 Expression Medium. The FreeStyle 293-F cell line is supplied in a vial containing 1mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle 293 Expression Medium and 10% DMSO
Characteristics of FreeStyle 293-F cells:
- Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were recloned by limiting dilution
- The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages
- Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle 293 Expression Medium
- Demonstrate high transfection efficiencies with FreeStyle MAX reagent
- Suspension cultures may be transfected in FreeStyle 293 Expression Medium without the need to change media
- Permits transfection of cells at large volumes
- Voltage limit: 0.5-2.5kV
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection
Specifications
Specifications
| Cell Line | FreeStyle™ 293-F Cells |
| Content And Storage | 1 vial of 1 x 107 cells. Store in liquid nitrogen. |
| Cell Type | 293-F |
| Species | Human |
| For Use With (Application) | Protein Expression and Transfection |
| Media Adaptation | FreeStyle™ 293 Expression Medium |
| Product Line | Freestyle |
| Product Type | 293-F Cell |
| Quantity | 1 mL |
| System Type | FreeStyle™ |
Frequently Asked Questions (FAQs)
Other 293 cell lines may be used with the FreeStyle 293 Expression System. However, they would have to be adapted to serum-free suspension culture in FreeStyle 293 Expression Medium and evaluated for transfection and expression.
When comparing FreeStyle 293-F cells with FreeStyle CHO-S cells for average range of antibody and protein yield, it is definitely expected that antibody yield will be higher in FreeStyle 293-F cells than in FreeStyle CHO-S cells, when generated by transient transfection. On the other hand, antibody yields in stable CHO cells can be much higher. For non-antibody proteins, it is impossible to predict which of the two cell lines may provide higher yields.
You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.
We typically keep the culture going for 7-10 days posttransfection. After that, protein expression may drop.
Our recommendation of 8% CO2 for the optimal growth of Freestyle 293 cells in Freestyle 293 Expression Medium is based on the bicarbonate content in the media and growth conditions of the cells observed by R&D. That said, you may use lower concentrations of CO2 if necessary (5% is common) to grow these cells. You may observe slightly slower growth kinetics or lower cell concentrations, but functionally the entire system will still work.
For Research Use Only. Not for use in diagnostic procedures.