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Invitrogen™ FreeStyle™ 293 Expression System

Catalog No. K900001
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K900001 1 Kit
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Catalog No. K900001 Supplier Invitrogen™ Supplier No. K900001
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Description

Includes

The FreeStyle™ 293 Expression System contains: 1L GIBCO FreeStyle 293 Expression Medium, 1 x 107 FreeStyle 293-F Cells (frozen), 1mL 293fectin Transfection Reagent, 100mL Opti-MEM™ I for Complex Formation, 25μg pCMV-SPORT β-gal

Complete, suspension cell culture system for generating large amounts of mammalian recombinant protein

  • Combines GIBCO™ FreeStyle 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle 293-F cells at high efficiency
  • FreeStyle 293-F cells and 293fectin are available as part of the Expression System or separately
  • Chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions
  • Complete, ready-to-use medium supplemented with animal-origin free GlutaMAX™-I Supplement
  • Saves time and costs associated with adaptation of cell cultures to serum-free conditions

Antibody & Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation & Analysis, Transfection, Transient Protein Production & High-Throughput Screening

Specifications

Cell Line FreeStyle™ 293-F Cells
Classification Serum-Free
Content And Storage The FreeStyle™ 293 Expression System contains:
  • 1L GIBCO™ FreeStyle™ 293 Expression Medium
  • 1 x 107 FreeStyle™ 293-F Cells (frozen)
  • 1 mL 293fectin™ Transfection Reagent
  • 100 mL Opti-MEM™ I for Complex Formation
  • 25 μg pCMV-SPORT β-gal
Please review the FreeStyle™ 293 System Manual for storage and handling information. Store FreeStyle™ 293-F Cells in liquid nitrogen. Store 293fectin™ Transfection Reagents at 4°C. Store GIBCO™ FreeStyle™ 293 Expression Medium and Opti-MEM™ I at 2-8°C in the dark. Store pCMV SPORT β-gal at -20°C. Reagents are guaranteed stable for six months when stored properly.
Delivery Type Transfection
Culture Type Suspension Cell Culture
Cell Type 293-derived Cells
Concentration 1 x 10^7 cells/mL
Expression Mechanism Cell-Based Expression
Expression System Mammalian
For Use With (Application) Protein Expression
Key Functions Protein Production
Product Line Freestyle
Product Type Expression System
Quantity 1 Kit
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For Research Use Only. Not for use in diagnostic procedures.