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Invitrogen™ FreeStyle™ 293 Expression System DFS Item

Complete, suspension cell culture system for generating large amounts of mammalian recombinant protein

Supplier:  Invitrogen™ K900001

Catalog No. K900001


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Includes: The FreeStyle™ 293 Expression System contains: 1L GIBCO FreeStyle 293 Expression Medium, 1 x 107 FreeStyle 293-F Cells (frozen), 1mL 293fectin Transfection Reagent, 100mL Opti-MEM™ I for Complex Formation, 25μg pCMV-SPORT β-gal

Description

Description

  • Combines GIBCO™ FreeStyle 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle 293-F cells at high efficiency
  • FreeStyle 293-F cells and 293fectin are available as part of the Expression System or separately
  • Chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions
  • Complete, ready-to-use medium supplemented with animal-origin free GlutaMAX™-I Supplement
  • Saves time and costs associated with adaptation of cell cultures to serum-free conditions

Antibody & Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation & Analysis, Transfection, Transient Protein Production & High-Throughput Screening

Specifications

Specifications

FreeStyle™ 293-F Cells
The FreeStyle™ 293 Expression System contains:
  • 1L GIBCO™ FreeStyle™ 293 Expression Medium
  • 1 x 107 FreeStyle™ 293-F Cells (frozen)
  • 1 mL 293fectin™ Transfection Reagent
  • 100 mL Opti-MEM™ I for Complex Formation
  • 25 μg pCMV-SPORT β-gal
Please review the FreeStyle™ 293 System Manual for storage and handling information. Store FreeStyle™ 293-F Cells in liquid nitrogen. Store 293fectin™ Transfection Reagents at 4°C. Store GIBCO™ FreeStyle™ 293 Expression Medium and Opti-MEM™ I at 2-8°C in the dark. Store pCMV SPORT β-gal at -20°C. Reagents are guaranteed stable for six months when stored properly.
Suspension Cell Culture
1 x 10^7 cells/mL
Mammalian
Protein Production
Expression System
Serum-Free
Transfection
293-derived Cells
Cell-Based Expression
Protein Expression
Freestyle
1 Kit
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For Research Use Only. Not for use in diagnostic procedures.