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Invitrogen™ DES™-Inducible Kit with pCoBlast

Catalog No. K512001
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K512001 1 kit
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Catalog No. K512001 Supplier Invitrogen™ Supplier No. K512001
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Description

Includes

Each kit contains 20μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2μg each), frozen S2 cells, GIBCO Schneider's Drosophila Medium, and the appropriate selection agent.

Small size (3.5kb) to improve DNA yields and increase subcloning efficiency

  • Provides expression vector pMT/V5-His and a Blasticidin (pCoBlast) selection
  • Uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to culture medium
  • C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody
  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
  • To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to coding sequence of C-terminal tag

Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Promoter MT
Product Type Inducible Kit
Content And Storage Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider's Drosophila Medium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Protein Tag Position (to your gene) C-terminal
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Gateway
Quantity 1 kit
Vector pMT
Product Line DES

Frequently Asked Questions (FAQs)

What is the doubling time of Drosophila S2 cells?

When grown at the optimal temperature of 27-28ºC, Drosophila S2 cells grow very quickly, with an approximate 14-16 hr doubling time. The cells can also be grown at 22-25ºC with doubling time of 24hrs. Humidity is an issue, so a shallow pan of water can be left in the incubator, but the incubator does not need to be turned on.

Growth medium is also a factor in doubling time - the doubling times listed here are for Sf900 II medium.

The cells can reach maximum densities of 1 to 2.5 x 10E7 cells/ml in shake flask cultures.

For Research Use Only. Not for use in diagnostic procedures.