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Thermo Scientific™ Imject™ Freund's Adjuvants

Stimulate a nonspecific immune response using complete (CFA) or incomplete (IFA) formulations of the popular Freund's adjuvant; offered in 10mL ampules.

Manufacturer:  Thermo Scientific™ PI77140

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Catalog No. PI77140



Thermo Scientific™ Imject Freund's Adjuvants are 10mL ampules of complete (FCA) and incomplete (FIA) formulations of a popular nonspecific stimulator of the immune response for use in antibody production immunization protocols.

The water-and-oil emulsions (with and without inactivated Mycobacterium) greatly enhance immune responses to immunogens when mixed and injected with the prepared antigen. Typically, FCA is used for initial injections and FIA is used for booster injections. The ampules comprise conveniently sized aliquots that provide long shelf life.


  • Water-in-oil emulsion with or without killed Mycobacterium
  • Enhance immune response and antibody production for injected immunogens
  • Useful for immunization of mice, rabbits and other mammalian hosts
  • Requires emulsification with prepared immunogens

Freund's Complete Adjuvant (FCA), Part No. 77140:

  • Water-in-oil emulsion containing killed Mycobacterium
  • Greatly enhances immune response to immunogen
  • Typically used for initial injections
  • Disadvantages: difficult to mix with immunogen and can cause tissue necrosis at injection site

Freund's Incomplete Adjuvant (FIA), Part No. 77145:

  • Water-in-oil emulsion without Mycobacterium
  • Enhances immune response in booster injections
  • Typically used for subsequent boosts after initial injection with FCA
  • Disadvantage: difficult to mix with immunogen


Imject Freund's Complete Adjuvant (FCA)
Oil-in-water emulsion containing killed Mycobacterium cells
5 x 10mL
Mixture with 50mL of immunogen