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Invitrogen™ Image-iT™ Green Hypoxia Reagent
Description
Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased. Image-iT Green Hypoxia Reagent sustains its fluorescence when cells/tissue return to normal oxygen levels, allowing the cells/tissue to be fixed with minimal loss of fluorescent signal.
Features of Image-iT Green Hypoxia Reagent include:
- Indicates hypoxia in live cells by fluorescing in low oxygen environments
- Fixable—sustains fluorescent signal when cells/tissue return to normal oxygen levels
- Easy to use—just add to cell culture media and image
Image-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties makes it a highly useful tool for detecting cells and tissue under hypoxic conditions. Image-iT Green Hypoxia Reagent is a very sensitive oxygen detector. Unlike pimonidazole adducts that respond only to very low oxygen levels, Image-iT Green Hypoxia Reagent begins to fluoresce when atmospheric oxygen levels are less than 5%. It responds quickly to such environments, and the fluorescence is sustained after the oxygen levels return to normal. These properties make Image-iT Green Hypoxia Reagent an ideal tool for detecting hypoxic conditions around tumors, 3D cultures, spheroids, neurons, etc.
Order Info
- Store at -5 to -30°C. Protect from light.
- Stable for at least 6 months after receiving.
Specifications
Specifications
| Content And Storage | Store at -5 to -30°C. Protect from light. |
| Product Type | Reagent |
| Product Line | Image-iT |
| Quantity | 1 Vial |
| Reagent Type | Hypoxia Reagent |
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
Frequently Asked Questions (FAQs)
Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.
This could be due to storage of the product under anaerobic or low oxygen conditions. The Image-iT Green Hypoxia Reagent increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Image-iT Green Hypoxia Reagent.
The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.
The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.
You may use cell-permeable nuclear counterstains or surface labels, but avoid any general cytoplasmic stains that may interfere with the emission of the hypoxia reagents.
For Research Use Only. Not for use in diagnostic procedures.