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Invitrogen™ Human IL-1R2/IL-1RB ELISA Kit
ELISA
$880.00
Specifications
| Accession Number | P27930 |
|---|---|
| Assay Range | 4.12 to 3000 pg/mL |
| Assay Sensitivity | 5 pg/mL |
| Calibration Range | 5 pg/mL to 3000 pg/mL |
| Assay | Solid-phase sandwich ELISA |
Description
The Human Interleukin 1 Receptor 2 (Hu IL-1R2/IL-1RB) ELISA quantitates Hu IL-1R2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1R2. Principle of the method The Human IL-1R2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported.Specifications
| P27930 | |
| 5 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| Human | |
| Colorimetric Microplate Reader | |
| CD121b,CDw121b,IL-1 R-2,IL-1 RT-2,IL-1 RT2c,IL1RB,IL1R2 | |
| <10 | |
| HRP | |
| Biomarker-Related Pathways | |
| 2°C to 8°C | |
| IL1R2 | |
| 4 hrs 45 mins |
| 4.12 to 3000 pg/mL | |
| 5 pg/mL to 3000 pg/mL | |
| HRP | |
| Cytokines & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta , MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 7850 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Plasma 5 μL, Serum 5 μL, Supernatant 100 μL | |
| CD121b, CDw121b, IL-1 R-2, IL-1 RT-2, IL-1 RT2c, IL1RB, IL1R2 | |
| 1 hrs 20 mins |