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Invitrogen™ Human IL-18RB/IL-18RAP ELISA Kit
ELISA
$890.00
Specifications
| Accession Number | O95256 |
|---|---|
| Assay Range | 12.29 to 3000 pg/mL |
| Assay Sensitivity | 12 pg/mL |
| Calibration Range | 12 pg/mL to 3000 pg/mL |
| Assay | Solid-phase sandwich ELISA |
Description
The Human Interleukin 18 Receptor Beta (Hu IL-18RB) (IL-18RAP) ELISA quantitates Hu IL-18RB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-18RB. Principle of the method The Human IL-18RB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18.Specifications
| O95256 | |
| 12 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| Human | |
| Colorimetric Microplate Reader | |
| ACPL,CD218b,CDw218b,IL-18 R-beta,IL-18 RAcP,IL-18 Rbeta,IL-1 R-7,IL-1R7,IL-1 RAcPL,IL18RB | |
| <10 | |
| HRP | |
| Inflammation | |
| 2°C to 8°C | |
| IL18RAP | |
| 4 hrs 45 mins |
| 12.29 to 3000 pg/mL | |
| 12 pg/mL to 3000 pg/mL | |
| HRP | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 8807 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Plasma 50 μL, Serum 50 μL, Supernatant 100 μL | |
| ACPL, CD218b, CDw218b, IL-18 R-beta, IL-18 RAcP, IL-18 Rbeta, IL-1 R-7, IL-1R7, IL-1 RAcPL, IL18RB | |
| 1 hrs 20 mins |