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Invitrogen™ Human IL-18 Matched Antibody Pair

Catalog No. 501125418
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10 x 96 Tests
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501125418 10 x 96 Tests
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Catalog No. 501125418 Supplier Invitrogen™ Supplier No. BMS2672MST

Description

ELISA

The Human IL-18 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample diluent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-18. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Specifications

Accession Number Q14116
Antigen IL-18
Assay Range 78-5000 pg/mL
Applications ELISA
Assay Sensitivity 78 pg/mL
Classification Monoclonal
Conjugate Biotin, HRP
Gene Accession No. Q14116
Gene Alias cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320; IGIF; IL-18; IL-1g; IL1F4
Gene Symbols IL18
Immunoassay Kit Format Matched Antibody Pair
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Quantity 10 x 96 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3606
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Content And Storage -20°C
Gene Symbol IL18
Kit Contents Capture (coating) Antibody, Detection Antibody, SAV-HRP, Standard, Sample Diluent
Label or Dye Biotin
Storage Requirements -20°C
Target IL-18
Test Time 2 hr 10 min
Total Assay Time 24 hr
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