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Invitrogen™ Human IL-17B ELISA Kit

ELISA

Supplier:  Invitrogen™ EHIL17B

Encompass

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Catalog No. EHIL17B


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Description

Description

The Human Interleukin 17B (Hu IL-17B) ELISA quantitates Hu IL-17B in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-17B. Principle of the method The Human IL-17B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.
Specifications

Chemical Identifiers

Q9UHF5
0.3 ng/mL
HRP
Cytokines & Receptors
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
27190
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
UNQ516/PRO1031, IL-17 B, IL-20, NIRF, ZCYTO7
1 hrs 20 mins
Solid-phase sandwich ELISA
0.41 to 100 ng/mL
0.3 ng/mL to 100 ng/mL
ELISA
Plasma, Serum, Supernatant
Human
Colorimetric Microplate Reader
IL-17 B,IL-20,NIRF,ZCYTO7
<10
HRP
Inflammation
2°C to 8°C
IL17B
4 hrs 45 mins

Specifications

Q9UHF5
0.3 ng/mL
Solid-phase sandwich ELISA
ELISA
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
27190
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Inflammation
2°C to 8°C
IL17B
4 hrs 45 mins
0.41 to 100 ng/mL
0.3 ng/mL to 100 ng/mL
HRP
Cytokines & Receptors
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Human
Colorimetric Microplate Reader
IL-17 B,IL-20,NIRF,ZCYTO7
<10
HRP
RUO
Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
UNQ516/PRO1031, IL-17 B, IL-20, NIRF, ZCYTO7
1 hrs 20 mins
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