missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 88871176

Invitrogen™ Mouse IL-17AF (heterodimer) Uncoated ELISA Kit with Plates

Catalog No. 88871176
Encompass
Change view
Click to view available options
Quantity:
20 x 96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
88871176 20 x 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 88871176 Supplier Invitrogen™ Supplier No. 88871176

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

ELISA

The Mouse Interleukin 17AF (heterodimer) (IL-17AF) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-17AF(heterodimer). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

Specifications

Accession Number Q62386,Q7TNI7
Assay Range 16 to 2,000 pg/mL
Assay Sensitivity 16 pg/ml
Conjugate HRP
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Mouse
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16171, 257630
Gene Symbol Ctla-8,Ctla8,IL-17,IL-17 A,Il17
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 20 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target IL-17 AF
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
Show More Show Less