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  5. Invitrogen™ Human IL-10RB ELISA Kit

Invitrogen™ Human IL-10RB ELISA Kit

Catalog No. EHIL10RB
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96 Tests
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Catalog No. Quantity
EHIL10RB 96 Tests
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Description

ELISA

The Human Interleukin 10 Receptor Beta (Hu IL-10RB) ELISA quantitates Hu IL-10RB in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10RB. Principle of the method The Human IL-10RB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions. Monoclonal antibodies have been used to identify different surface antigens present on NK cells. These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets. The Cluster of Differentiation (CD) nomenclature was established to standardize the naming of NK cells.

Specifications

Accession Number Q08334
Assay Range 5-3000 pg/mL
Assay Sensitivity 5 pg/mL
Calibration Range 5 pg/mL to 3000 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Cytokines & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 6620401D04Rik; AI528744; CDW210B; CRF2-4; CRFB4; cytokine receptor class-II CRF2-4; cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; cytokine receptor family II, member 4; D16H21S58; D21S58; D21S58h; D21S66; IL-10 receptor subunit beta; IL-10R subunit 2; IL-10R subunit beta; Il10r2; IL-10R2; IL10RB; IL-10RB; inte; interleukin 10 receptor 2; interleukin 10 receptor subunit beta; interleukin 10 receptor, beta; interleukin-10 receptor subunit 2; Interleukin-10 receptor subunit beta; RGD15603736620401D04Rik; AI528744; CDW210B; CRF2-4; CRFB4; cytokine receptor class-II CRF2-4; cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; cytokine receptor family II, member 4; D16H21S58; D21S58; D21S58h; D21S66; IL-10 receptor subunit beta; IL-10R subunit 2; IL-10R subunit beta; Il10r2; IL-10R2; IL10RB; IL-10RB; inte; interleukin 10 receptor 2; interleukin 10 receptor subunit beta; interleukin 10 receptor, beta; interleukin-10 receptor subunit 2; Interleukin-10 receptor subunit beta; RGD1560373; CDW210B; CRF2-4; CRFB4; D21S58; D21S66; IL-10R2
Gene ID (Entrez) 3588
Gene Symbol IL10RB
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Signal Transduction
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2
Target IL10RB
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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