missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. Primary Antibody Matched Pairs
  6. CHC1323

Invitrogen™ Human IL-10 Matched Antibody Pair

Catalog No. CHC1323
Encompass
Change view
Click to view available options
Quantity:
10 x 96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
CHC1323 10 x 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. CHC1323 Supplier Invitrogen™ Supplier No. CHC1323

Description

ELISA

The Human IL-10 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-10. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

Accession Number P22301
Assay Range 31.2-2000 pg/mL
Assay Sensitivity 31.2 pg/mL
Conjugate HRP
Gene Accession No. P22301
Gene Alias CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; GVHDS; IL-10A; TGIF; IL10
Gene Symbols IL10
Immunoassay Kit Format Matched Antibody Pair
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Quantity 10 x 96 Tests
Target Species Human
Gene ID (Entrez) 3586
For Use With (Equipment) Absorbance Microplate Reader
Includes Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Content And Storage Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gene Symbol IL10
Kit Contents Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Label or Dye Biotin
Protein Aliases IL-10, Cytokine synthesis inhibitory factor, CSIF
Regulatory Status RUO
Shipping Condition Wet Ice
Storage Requirements 2-8°C
Target IL-10
Test Time 1 hr 30 min
Total Assay Time 24 hr
Show More Show Less