missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 885054086

Invitrogen™ Rat IgM Uncoated ELISA Kit with Plates

Catalog No. 885054086
Encompass
Change view
Click to view available options
Quantity:
10 x 96 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
885054086 10 x 96 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 885054086 Supplier Invitrogen™ Supplier No. 885054086

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

ELISA

The Rat IgM Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IgM. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens, such as bacteria and viruses. Rat IgM has been extensively studied and is widely used in research applications, including immunology, microbiology, and biotechnology. It is commonly used as a control in experiments, as well as a diagnostic tool for detecting infectious diseases and autoimmune disorders. Researchers have developed various techniques for producing and purifying Rat IgM, including hybridoma technology, protein A/G chromatography, and affinity chromatography. Rat IgM is a valuable tool in the field of immunology, and its use has contributed to a better understanding of the immune system and the development of new treatments for various diseases.

Specifications

Assay Range 0.3125 to 20 ng/mL
Assay Sensitivity 0.3125 ng/ml
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Rat
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 299357
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Storage Requirements 2°C to 8°C
Target Rat IgM
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
Show More Show Less