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  5. Invitrogen™ Mouse IgE ELISA Kit

Invitrogen™ Mouse IgE ELISA Kit

Catalog No. EMIGHE
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Quantity:
96 Tests
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Catalog No. Quantity
EMIGHE 96 Tests
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Description

ELISA

The Mouse Immunoglobulin E (Ms IgE) ELISA quantitates Ms IgE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IgE. Principle of the method The Mouse IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.

Specifications

Accession Number N/A
Assay Range 0.137 to 100 ng/mL
Assay Sensitivity 0.14 ng/mL
Calibration Range 0.14 ng/mL to 100 ng/mL
Description IgE ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Other Proteins Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of IgE in Samples
Detectable Analytes Mouse IgE
Assay 96
Conjugate HRP
Detection Method Colorimetric
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects mouse IgE. Other species not determined.
Substrate Type TMB
Sufficient For 96 Assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 380792
Host Species Mouse
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Immunology
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym N/A
Target Mouse IgE
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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