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  5. Invitrogen™ Human IgA ELISA Kit

Invitrogen™ Human IgA ELISA Kit

Catalog No. 501125318
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10 x 96 Tests
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501125318 10 x 96 Tests
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Catalog No. 501125318 Supplier Invitrogen™ Supplier No. BMS2096TEN
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Description

ELISA

The Human ImmunoGlobulin A (Hu IgA) ELISA quantitates Hu IgA in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgA. Principle of the method The Human IgA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgA, also known as human immunoglobulin A, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in mucosal secretions, such as saliva, tears, and breast milk, and plays a key role in defense against pathogens at mucosal surfaces. IgA works by binding to specific antigens and preventing them from entering the body's tissues. It can also activate complement and promote phagocytosis by immune cells. Human IgA can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious diseases.

Specifications

Accession Number P01876, P01877
Assay Range 1.6-100 ng/mL
Assay Sensitivity 0.03 ng/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human IgA
HRP-Conjugate anti-human IgA monoclonal antibody
human IgA Standard lyophilized, 200 ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IgA1; IGHA1; IGHA2; Immunoglobulin A; Immunoglobulin Am1; Immunoglobulin Am2; immunoglobulin heavy constant alpha 1; Immunoglobulin Heavy Constant Alpha 2; immunoglobulin heavy constant alpha 2 (A2m marker)A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IgA1; IGHA1; IGHA2; Immunoglobulin A; Immunoglobulin Am1; Immunoglobulin Am2; immunoglobulin heavy constant alpha 1; Immunoglobulin Heavy Constant Alpha 2; immunoglobulin heavy constant alpha 2 (A2m marker);
Gene ID (Entrez) 3493, 3494
Gene Symbol IGHA1, IGHA2
Interassay CV 4.8%
Intraassay CV 3.9%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma, 20 μL; Serum, 20 μL
Storage Requirements 2-8°C
Synonym Immunoglobulin A
Target Human IgA
Test Time 1 hr 20 min
Total Assay Time 1 hr 30 min
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