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Invitrogen™ Human IFN gamma ELISA Kit

Numéro de catalogue. KAC1231
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Quantité:
96 Tests
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Numéro de catalogue. Quantité
KAC1231 96 Tests
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Numéro de catalogue. KAC1231 Fournisseur Invitrogen™ Code fournisseur KAC1231

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Description

ELISA

The Human Interferon-gamma (Hu IFN-γ) ELISA quantitates Hu IFN-γ in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFN-γ. Principle of the method The Human IFN-γ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Spécifications

Numéro d'enregistrement P01579
Plage de l'essai 1-30 IU/mL
Sensibilité d’essai 0.03 IU/mL
Contenu et stockage Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Conjugué HRP
Format de la trousse d’immunodosage Sandwich ELISA Kit
Type de produit ELISA
Famille de protéines Cytokines & Receptors
Type d’échantillon Plasma, Serum, Supernatant
Espèces cibles Human
À utiliser avec (application) Colorimetric Detection
À utiliser avec (équipement) Absorbance Microplate Reader
Alias du gène BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-gBoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g; IFG; IFI
ID du gène (Entrez) 3458
Symbole de gène IFNG
Temps d’incubation 2.25 hr.
CV intra-essai 7.3%, 3.5%
Contenus des kits Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
Étiquette ou colorant HRP
Marqueur génétique IFN-γ
Alias des protéines IFN-gamma, Immune interferon
Quantité 96 Tests
Statut réglementaire RUO
Volume d’échantillon Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping condition (Condition d'expédition) Wet Ice
Conditions de stockage 2-8°C
Cible IFN gamma
Durée du test 1 hr 20 min
Durée totale de l'essai 2 hr 15 min
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