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Invitrogen™ iBright™ FL1500 Imaging System

Catalog No. A44115
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Software:
iBA Analysis Software
SAE software and iBA Secure software
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1 Year Warranty
2 Year Extended Warranty
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Catalog No. Software Includes
A44115 iBA Analysis Software 1 Year Warranty
A44241 iBA Analysis Software 2 Year Extended Warranty
A44241CFR SAE software and iBA Secure software 2 Year Extended Warranty
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Catalog No. A44115 Supplier Invitrogen™ Supplier No. A44115

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Capture images and analyze data from western blots and gels efficiently and easily with the Invitrogen iBright FL1500 Imaging System. This high-performance instrument enhances the imaging experience through powerful hardware, advanced automated technologies, and an interface that is easy to use for researchers of all experience levels.

Capture images and analyze data from western blots and gels efficiently and easily with the Invitrogen iBright FL1500 Imaging System. This high-performance instrument enhances the imaging experience through powerful hardware, advanced automated technologies, and an interface that is easy to use for researchers of all experience levels.

The iBright FL1500 Imaging System supports the main imaging applications of fluorescent, chemiluminescent, and colorimetric western blots, in addition to fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. We also offer the iBright CL1500 Imaging System, which features the same imaging capabilities as the iBright FL1500 Imaging System, but without fluorescent western blot imaging functionality.

Features of the iBright FL1500 Imaging System include:

  • 9.1 MP cooled CCD camera—high sensitivity and dynamic range to help enable the detection of subtle differences in samples
  • Five fluorescence channels—multiplex and capture up to four proteins in a single blot for more meaningful and representative experiments
  • Smart Exposure technology—provides rapid determination of optimal exposure time to help minimize the need to repeat exposures to acquire the desired signal
  • High Dynamic Range Imaging*—Smart Range HDR combines optimized short and long exposures into a single HDR image to capture an extended linear dynamic range and providing high-quality non-saturated data for improved quantitation
  • Simple interface—clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
  • Advanced automated features—automatic sample rotation, automatic zoom, and automatic focus help streamline image capture
  • 22.5 x 18.0-cm field of view—large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
  • Green LED-based transilluminator—effectively excite popular DNA dyes such as ethidium bromide and Invitrogen SYBR Green dyes with an alternative to UV-based transilluminators
  • Expanded imaging applications*—capture and analyze visible, chemiluminescent or fluorescent stained colonies on single- or multi-well cell culture plates using the optimized imaging channels and onboard analysis workflows
  • Flexible connectivity—export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to Connect cloud-based platform
  • Multiple image analysis options—perform densitometry, quantitation, and normalization directly using the on-instrument software, or for more in-depth analysis use iBright Analysis Software (available in both desktop and cloud-based versions)

*Certain functions are made available through firmware. Ensure your system is running the most up to date firmware version.

Specifications

Includes 1 Year Warranty
Resolution 9.1 MP
Software iBA Analysis Software
Model FL1500
Camera Type CCD
Content And Storage Imaging Sample Blot, Preinstalled filter sets, Safe Imager Viewing Glasses, White Screen for stained SDS-PAGE gel analysis, iBright Imager Quick Start Guide
Dimensions 68 x 38 x 60 cm
Format Image formats: G2i (proprietary), TIFF, JPG, PNG, PDF
Weight 50 kg (110 lb.)
Field 22.5 cm x 18 cm (WxD)
Shipping Condition Room Temperature
For Use With (Application) Chemiluminescent Western Blot Imaging
Product Line iBright
Product Type Imaging System
Quantity 1 system
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Frequently Asked Questions (FAQs)

Do the iBright imagers support total protein normalization?

iBright Imaging Systems support total lane protein-based normalization and housekeeping gene (HKP)-based normalization. To support the methods best suited for your experiment, iBright Imaging Systems and iBright Analysis Software provide multiple quantitation and normalization options to monitor or mathematically compensate for experimental or sample variability.

To learn more about total lane protein normalization, explore our application note.
Are the iBright Imaging Systems network-compatible?

The iBright Imaging Systems support Ethernet network connections. WIFI is also supported with the addition of a USB-WIFI dongle. Data may be exported from the instrument via network and WIFI connections. It can also be exported via USB port.

For additional information on iBright functionality, visit this page.
What fluorophores can I image with the iBright FL1500 Imaging System?

The iBright FL1500 Imaging System supports a wide range of dyes, stains and fluorophores in the visible and NIR wavelength range.

For more information on compatible reagents and applications, visit this page.

To learn more about the iBright system-supported fluorophores and installed filters and light sources, visit Fluorescence SpectraViewer.
Is a computer needed to control the iBright Imaging Systems?

iBright Imaging Systems feature a built-in computer interface controlled by the 12.1 inch touchscreen. No additional computer is needed to operate the instrument.

For additional information on iBright functionality, visit this page.
Why do the iBright Imaging Systems use green LED illumination for DNA imaging?

The iBright Imaging Systems utilize a transilluminator based on green LEDs, which effectively excite popular DNA dyes such ethidium bromide and SYBR Green dye and offer several additional benefits:

- No harmful UV rays: While UV light effectively excites many fluorescent dyes and stains, UV light is a health hazard. Further, prolonged exposure to UV light can damage DNA samples, and compromise the integrity of samples to be used for downstream applications, such as subcloning.
- No mercury waste: UV transilluminator bulbs may contain mercury, a hazardous substance, and therefore require special care for handling and disposal.
- Longer lifetime: LED bulbs have a substantially longer real-time life than fluorescent UV bulbs, which can add up to considerable cost savings over the lifetime of the instrument.

For additional information on iBright functionality, visit this page.
What automation features are included with the iBright FL1500 and CL1500 Imaging Systems?

The iBright FL1500 and CL1500 Imaging Systems include a suite of powerful automation tools to facilitate easy image capture and improve laboratory efficiency. These features include:
- Smart Exposure: this technology rapidly determines the optimal exposure time, minimizing the potential for over- or under-exposed images and the need to repeat exposures to get the desired signal. Smart exposure is enabled by built-in algorithms which aim to maximize sensitivity without creating band saturation.
- Automatic Mechanical Sample Rotation: The imager senses the edge of the sample and will physically rotate the turntable up to 10 degrees to straighten the sample prior to imaging.
- Automatic Zoom and Focus: After the sample is inserted in the imager, the system automatically optimizes the zoom and focus levels to allow for fast easy image capture and optimized image resolution.

For additional information on iBright functionality, visit this page.
How many different iBright Imaging System models do you offer?

We offer three different iBright Imaging System models, the iBright FL1500, CL1500, and CL750 iBright Imaging Systems.

To learn more about the capabilities of these three iBright imager models, visit this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
Do the iBright Imaging Systems support .tiff file export?

iBright imagers support the export of .tiff files in 8 and 16 bit formats. They also allow users to export images as .jpg and the proprietary .g2i file types. The iBright Analysis software primarily uses .g2i files as this format contains the most data to facilitate easy analysis. But it also supports .tiff files import and analysis.

For additional information on iBright functionality, visit this page.
What dyes and stains are supported by the iBright Imaging Systems?

The iBright Imaging Systems support a wide range of dyes, stains, and fluorophores from the visible to NIR wavelength range.

For more information on compatible reagents and applications, visit this page.

To learn more about the iBright system-supported fluorophores and installed filters and light sources, visit Fluorescence SpectraViewer.
How large is the sample imaging area on the iBright Imaging Systems?

The imaging area of the iBright Imaging system is 22.5 x 18.0 cm. This gives ample space for imaging 4 mini blots (or 2 midi blots) simultaneously.

For more information on iBright features and capabilities, visit this page.
How large is the touchscreen on the iBright imager?

iBright imagers include a 12.1 inch LCD touchscreen interface.
Is analysis software included with purchase of an iBright Imaging System?

The free iBright Analysis Software (iBA) supports analysis of all images captured on iBright imagers and is available for free download on our website. There is also a Cloud version of iBA software available for users who prefer to work directly from a web browser.

The iBA software is supported on both Windows and Mac computers and can be used by any lab personnel who need it. This software automates data analysis and makes annotation, analysis, and reporting simple. To learn more about the iBA software, view the iBright Analysis Software how-to-videos available on this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
What sample types can I image with the iBright FL1500 Imaging System?

The iBright FL1500 Imaging System provides maximum application support including up to 4-channel multiplex western blot imaging. See the list below for more details:

Protein gel: colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)

Nucleic acid gel: ethidium bromide and Invitrogen SYBR stains; imaging of E-Gels and hand-poured gels is supported

Chemiluminescent western blot: chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)

Fluorescent western blot: fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates)

Additional applications: colony counting for GFP, crystal violet, and blue/white colonies; TLC plates, and leaf sections

For additional information about these applications or to learn more about the three iBright model capabilities, visit this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
What are the primary differences between the iBright SAE secure version of the iBright Analysis software and the standard version of the software?

Most functionality is the same between the standard and secure versions of the iBright Analysis (iBA) software. The primary difference is that the secure version of the software supports the auditing and signature functionality that is needed to support 21CFR laboratories.

To learn more about iBA software, visit this page.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
What analysis software is compatible with iBright Imaging Systems?

iBright Analysis Software (iBA) is available for free download on our website. There is also a Cloud version of iBA software available for users who prefer to work directly from a web browser.

The iBA software is supported on both Windows and Mac computers and can be used by any lab personnel who need it. This software automates data analysis and makes annotation, analysis, and reporting simple. To learn more about the iBA software, view the iBright Analysis Software how-to-videos available on this page.

iBright imagers also support 16 bit .tiff export; this file type can be used with other second party software programs if desired.

To speak with a representative about the iBright imager or request a quote or demo, please fill out this form.
How are images on an iBright Imaging System printed on a thermal printer?

Once communication is established between the IBright imager and the thermal printer, a print button will become available on the main screen near the image display. Pressing this button will initiate printing of the image currently shown on the instrument screen. This is a separate workflow from the print settings available through the system menu, which are used for printing image reports on a standard network printer.

For more information on iBright features and capabilities, visit this page.
How does the Thermal Printer for iBright Imaging Systems (Cat. No. A45235) interface with the iBright Imaging Systems?

To establish communication between the Thermal Printer and the iBright imager, first the communication channel on the printer needs to be updated. This can be done by accessing the printer menu, navigating from Digital to Driver and then using the up down toggle to set the printer to channel 897. Once that is complete, the thermal printer can then be connected directly to one of the iBright imager rear USB ports via A to B type USB cable. After installation, the thermal printer can be controlled directly with the iBright onboard software via the on-screen print functionality.
Is the iBright System Contrast Tray (Cat. No. A56597) included with purchase of an iBright imager?

The iBright System Contrast Tray is included with the purchase of a current iBright Imaging System, inclusive of the CL750, CL1500, and FL1500 iBright Imaging Systems. For additional information on iBright features and capabilities, visit the iBright Imaging Systems page.
Which iBright imager models are compatible with the iBright System Contrast Tray (Cat. No. A56597)?

All iBright imager models are compatible with the orange iBright System Contrast Tray (Cat. No. A56597), inclusive of the CL1000, FL1000, CL750, CL1500, and FL1500 iBright imager models. For more information on compatible reagents and applications, visit the iBright Imaging Systems Compatible Reagents page.
What sample types can I image with the iBright System Contrast Tray (Cat. No. A56597)?

We recommend using the iBright System Contrast Tray (Cat. No. A56597) for imaging all visible protein gel samples such as those stained with Coomassie Blue or Silver Stain. For more information on compatible reagents and applications, visit the iBright Imaging Systems Compatible Reagents page.
What is the best way to image Ponceau staining with an iBright Imaging System?

The recommended imaging method depends on the iBright imager model being used. For iBright FL1500 and CL1500 Imaging Systems, we recommend imaging Ponceau-stained samples with the Ponceau application that can be found in Universal mode. For the iBright CL750 Imaging System, we recommend imaging Ponceau-stained samples in Protein gel mode.

We always recommend imaging with the orange iBright System Contrast Tray (Cat. No. A56597), and if needed, adjust the focus or zoom to get bands as sharp as possible. Better quality pictures are obtained when the membrane is wet.
Upon trying to install the Invitrogen iBright desktop software on my MacBook, I receive a notification that says "software can't be opened because Apple cannot check it for malicious software. This software needs to be updated. Contact developer."

In order to install the iBright software, the user should be an admin.

  • Open download folder and double click iBrightAnalysisSoftware.zip to extract files.
  • Right click on the iBrightAnalysisSoftware.pkg and open it.
    • Note: You will see a pop‐up window if Xcode software command line tool is not installed on your system.
  • Accept Xcode license and install the software.
  • Accept the terms and conditions (End User Licensing Agreement)
  • Click on Install.
  • A prompt will ask your password for installation of application.
  • Launch the application from Applications folder.
What is the weight of the Thermal Printer for iBright Imaging Systems?

The Thermal Printer for iBright Imaging Systems weighs approximately 5.5 lbs (2.5 kg).
What are the dimensions of the Thermal Printer for iBright Imaging Systems?

Here are the dimensions of the Thermal Printer for iBright Imaging Systems:
Approximately 3.46 x 6.06 x 9.45 inches (88 x 154 x 240 mm).
What is the power consumption of the Thermal Printer for iBright Imaging Systems?

In stand-by mode, the power consumption of the Thermal Printer for iBright Imaging Systems is 10 Watts, and during the printing process, it is 115 Watts.

Which computer systems are compatible with the Thermal Printer for iBright Imaging Systems?

Windows 7/8/10 (32/64 bit) and MacOSX systems are compatible with the Thermal Printer for iBright Imaging Systems.
What type of paper do you recommend for the Thermal Printer for iBright Imaging Systems?

We recommend using UVP High Gloss Thermal Printer Paper (Fisher Cat. No. UVP89017401). It is 110 mm wide, with 220 to 245 prints per roll.

Are you planning to develop additional data analysis software for the iBright imager?

The iBright imager now has software enabling Total Protein Normalization when using the No-Stain Protein Labeling Reagent. Additional data analysis software for the iBright imager is not being planned at this time.
How can I image Ponceau staining best using an iBright imager?

We recommend taking Ponceau staining images in “Protein gel” mode, with the white screen. It is possible to play with the focus/resolution/sensitivity to get bands as sharp as possible. Better quality pictures are obtained when the membrane is wet.

How does the smart exposure technology work for an iBright imager?

Smart exposure technology rapidly determines optimal exposure time, which helps minimize the potential for over- or underexposed images, and the need to repeat exposures to get the desired signal. Smart exposure is enabled by built-in algorithms which aim to maximize sensitivity without creating band saturation. Smart exposure time varies depending on the gel imaged and bands displayed.

What is the difference between the iBright CL1500 Imaging System and the iBright FL1500 Imaging System?

The iBright CL1500 Imaging System allows imaging of chemiluminescent Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and visible protein gels. The iBright FL1500 Imaging System adds the capability of imaging fluorescent Western blots using multiple dyes.

For Research Use Only. Not for use in diagnostic procedures.