missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. General Western Blot Reagents
  5. Invitrogen™ iBind™ Flex Solution Kit

Invitrogen™ iBind™ Flex Solution Kit

Catalog No. SLF2020X4
Encompass
Change view
Click to view available options
Quantity:
1 kit
4 kits
Product Type:
Flex Solution Kit
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity Product Type
SLF2020X4 4 kits Flex Solution Kit
SLF2020 1 kit Flex Solution Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. SLF2020X4 Supplier Invitrogen™ Supplier No. SLF2020X4
Only null left

Description

Detection kit for use with iBind™ Flex Western System

• Optimized solutions with ideal viscosity for blocking, washing, and antibody dilution
• Long shelf life—store iBind Flex Solution Kit for 12 months at 4°C
• Better antigen-antibody interaction—solutions flow between the iBind Flex Card and membrane for maximum interaction between protein and antibody

Specifications

Product Line iBind
Membrane Compatibility Nitrocellulose, PVDF
Detection Method Colorimetric, Chromogenic, Chemiluminescence
Product Type Flex Solution Kit
Content And Storage iBind Flex 5X buffer (400 mL)
iBind Flex 100X additive (12 x 1.7 mL)

Store at 2–8°C.
No. of Reactions 10 midi blots or 20 mini blots
Shipping Condition Qualified for Ambient or Wet Ice
For Use With (Equipment) iBind Flex Western Device
Quantity 4 kits
For Use With (Application) Western Blotting

Frequently Asked Questions (FAQs)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.
Is it easy to change the well inserts on the iBind Flex Western Device?

Yes. Due to an intuitive magnetic design, it is very simple for the user to change the well inserts. However, we highly recommend the user handle the well inserts with care (as engraved on each insert) when changing or washing, and store the other two well inserts in the convenient underside tray when not in use.
Is there any difference between the iBind Flex Card and the original iBind Card?

Besides being ~1.7 times as wide, the iBind Flex Card also includes alignment lines printed on the back that appear when solution is added to the card to help in proper horizontal placement of multiple mini or vertically cut strip blots.
Is there any difference between the iBind Flex Solution Kits and the original iBind Solution Kits?

The formulations are identical for both the standard and Fluorescent Detection (FD) solutions. The only difference is in the amount supplied, so the iBind Flex Solution Kits are sufficient for 10 midi, 20 mini, or 60 vertically cut strip blots.
Is the iBind Flex Western Device compatible with horizontally cut strip blots that are rotated 90 degrees and processed vertically?

It is not recommended due to the potential for non-uniform flow from top to bottom which could result in a gradient signal intensity across horizontally cut strip blots.
Does the original iBind Card fit on the iBind Flex Western Device?

Yes, to run a single mini blot or up to three vertically cut strip blots. However, when using the Mini or Multi-Strip Inserts, wells not in contact with the card should remain empty.
Can you run only one mini blot or less than six vertically cut strip blots on the iBind Flex Western Device when using the iBind Flex Card?

Yes. Whether using the Mini or Multi-Strip Inserts, empty wells are still in contact with the card and should be filled with water to ensure proper flow of all solutions across the card.
For the iBind Flex Western System, do you have a protocol for detecting the signal using the LI-COR Odyssey Imaging System?

Please use the protocol mentioned on Page 15 of the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/ibind_flex_man.pdf) when using the iBind Flex Western System in conjunction with the LI-COR Odyssey Imaging System. We do not recommend using the LI-COR solution.
What iBind Flex Solution kits are available?

We offer the iBind Flex Solution kit and the iBind Flex Fluorescent Detection (FD) Solution kit. For standard chemiluminescent or chromogenic detection, we recommend the iBind Flex Solution kit (Cat. Nos. SLF2020, SLF2020X4) and for fluorescent detection, we recommend the iBind Flex Fluorescent Detection (FD) Solution kit (Cat. No. SLF2019).
When is it necessary to pre-activate a PVDF membrane with methanol for use in the iBind Flex Western Device?

If the PVDF membrane has dried up, it is important to pre-activate it with 100% methanol and then rinse in distilled water before immersing it in iBind Flex Solution/iBind Flex Fluorescent Detection (FD) Solution. It is not necessary to activate a PVDF membrane that has just come out of the transfer and moved into iBind Flex Solution/iBind Flex Fluorescent Detection (FD) Solution.
What are your recommendations for cleaning and maintenance of the iBind Flex Western device?

- Rinse the iBind Flex well inserts under running water after each use and allow the well inserts to dry before additional usage.
- Handle well inserts with care.
- Store unused well inserts in the drawer in the iBind Flex Western Device.
- To maximize the life of the springs in iBind Flex Western Device, store the device with latch unlocked, and the lid open as shown on Page 28 of the manual (https://tools.thermofisher.com/content/sfs/manuals/ibind_flex_man.pdf).
What is the recommended amount of time to immerse the membrane in iBind Flex Solution/iBind Flex Fluorescent Detection (FD) Solution prior to assembly in the iBind Flex Western device?

It is sufficient to block the membrane in iBind Flex Solution/ iBind Flex Fluorescent Detection (FD) Solution for the time prior to making antibody dilutions. It is not necessary to block the membrane overnight in the blocking solution. The membrane will be subjected to additional blocking in the iBind Solution/ iBind Flex Fluorescent Detection (FD) Solution before primary antibody reaches the membrane.
Can I use my own blocking reagent or antibody incubation solution with the iBind Flex Western System?

We do not recommend using your own solutions. The iBind Flex Solution and iBind Flex Fluorescent Detection (FD) Solution have specific viscosity and are optimized for the sequential lateral flow that is the principle of the iBind Flex Western device. We cannot guarantee the performance with any other solutions.
What are the components of the iBind Flex Western System and which components do I need to purchase separately?

The iBind Flex Western System (Cat. No. SLF2000) consists of:
- iBind Flex Western Device
- iBind Flex Midi Insert
- iBind Flex Mini Insert
- iBind Flex Multi-Strip Insert
- iBind Flex Blotting Roller

You would need to purchase the following items separately:
- iBind Flex Cards (Cat. No. SLF2010)
- iBind Flex Solution Kit (Cat. No. SLF2020) for preparing blocking, dilution, and washing buffers for chemiluminescent or chromogenic detection
- iBind Flex Fluorescent Detection Solution Kit (Cat. No. SLF2019) for preparing blocking, dilution, and washing buffers for fluorescent detection
- Invitrogen AP Mouse Chemiluminescent Detection Kit (Cat. No. SLF1021) or Invitrogen AP Rabbit Chemiluminescent Detection Kit (Cat. No. SLF1022)
How do iBind Western Systems work?

iBind Western Systems are benchtop devices that automate immunodetection steps. These devices use no external power source, and rely on mechanical pressure to generate sequential lateral flow (SLF) of immunodetection reagents to perform hands-free blocking, antibody binding, and washes for western detection workflows.

Two iBind Western Systems are available:
i) containing the original iBind Western Device, which accommodates one mini blot at a time
ii) containing the iBind Flex Western Device, which accommodates one or two mini blots, one midi blot, or up to six vertically cut membrane strips (for separate antibodies) at a time.

For Research Use Only. Not for use in diagnostic procedures.