Thermo Scientific™ I-PER™ Insect Cell Protein Extraction Reagent

Description

Thermo Scientific™ I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture.

The baculovirus insect cell expression system is an efficient and popular system for the production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

Highlights:

• Gentle extraction – optimized, mild non-ionic detergent provides maximum extraction of soluble proteins from insect cells

• Effective – provides better protein extraction than sonication

• Compatible – downstream compatibility with Western blotting, 6xHis-tagged protein purification, protein assays and ion-exchange chromatography

• Flexible – useful for protein extraction from suspended or adherent cultured insect cells