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Invitrogen™ FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit

Catalog No. 501138077
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Catalog No. 501138077 Supplier Invitrogen™ Supplier No. F34953
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Description

FungaLight™ Yeast CFDA, AM/Propidium Iodide Vitality Kit

The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Specifications

Cell Type Yeast Cells
Content And Storage Contains 3 vials of 5-CFDA (1 mg per vial), 1 vial of propidium iodide, and 1 vial of DMSO. Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type AM/Propidium Iodide Vitality Kit
Dye Type Other Label(s) or Dye(s)
Emission PI: 490/635, CFDA: 492/517
Form Solid
Format Tube(s), Slide(s)
Product Line FungaLight
Quantity 200 kit
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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Frequently Asked Questions (FAQs)

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

For Research Use Only. Not for use in diagnostic procedures.