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Molecular Probes™ BacLight™ RedoxSensor™ Green Vitality Kit

Catalog No. B34954
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B34954 200 kit
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Catalog No. B34954 Supplier Molecular Probes™ Supplier No. B34954
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Description

BacLight™ RedoxSensor™ Green Vitality Kit

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for changes in vitality that occurs following antibiotic treatment. RedoxSensor™ Green reagent penetrates both gram-positive and gram-negative bacteria. Following reduction, the RedoxSensor™ Green reagent will produce a stable green-fluorescent signal in 10 minutes that is compatible with formaldehyde fixation techniques.

Specifications

Cell Type Bacteria
Content And Storage Contains 1 vial of RedoxSensor™ green (200 μL, 1 mM solution in DMSO), 1 vial of propidium iodide (300 μL, 20 mM solution in DMSO), 1 vial of sodium azide (1 mL of a 2 M solution in water), and 1 vial of CCCP (400 μL, 5mM in DMSO).
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Green Vitality Kit
Dye Type RedoxSensor™ green, PI
Emission PI: 490/635, RedoxSensor™ green: 490/520
Form Solution
Format Tube(s), Slide(s)
Product Line BacLight, RedoxSensor
Quantity 200 kit
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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Frequently Asked Questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).
What is the excitation/emission of the RedoxSensor Green reagent?

The excitation/emission of the RedoxSensor Green reagent, in reduced form, is 490/520 nm.

For Research Use Only. Not for use in diagnostic procedures.