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Invitrogen™ BacLight™ RedoxSensor™ CTC Vitality Kit

Catalog No. 361045886
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Catalog No. 361045886 Supplier Invitrogen™ Supplier No. B34956
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Description

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures.

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.

Specifications

Cell Type Bacteria
Content And Storage • 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), 5 x 15 mg vials
• SYTO™ 24 green (100 μL in DMSO), 1 vial
• DAPI (5 mg/mL solution in water), 100 μL

Store in freezer (-5°C to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type CTC Vitality Kit
Dye Type Other Label(s) or Dye(s)
Emission 488
Format Tube(s), Slide(s)
Product Line BacLight, RedoxSensor
Quantity 1 Kit
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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Frequently Asked Questions (FAQs)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

For Research Use Only. Not for use in diagnostic procedures.