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  5. Invitrogen™ Human YWHAE ELISA Kit

Invitrogen™ Human YWHAE ELISA Kit

Catalog No. EH487RBX5
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Description

ELISA

Human YWHAE quantitates human YWHAE in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human YWHAE.

14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Specifications

Accession Number 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3 E; AU019196; epididymis luminal protein 2; HEL2; KCIP-1; MDCR; MDS; mitochondrial import stimulation factor (MSF) L subunit; mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; YWHAE
Assay Range 0.34 to 80 ng/mL
Assay Sensitivity 0.34 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7531
Gene Symbol YWHAE
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target 14-3-3 epsilon
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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