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Invitrogen™ Human Visfatin ELISA Kit

Catalog No. EH482RBX5
Encompass
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5 x 96 Tests
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EH482RBX5 5 x 96 Tests
EH482RB 96 Tests
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ELISA

Human Visfatin quantitates human Visfatin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Visfatin.

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.

Specifications

Accession Number 1110035O14Rik; AI314458; AI480535; DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nampta; nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase a; PBEF; Pbef1; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; RCJMB04
Assay Range 1.1 to 300 ng/mL
Assay Sensitivity 1.1 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 10135
Gene Symbol NAMPT
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target NAMPT
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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