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Invitrogen™ Human VGAT (aa 166-207) Control Fragment Recombinant Protein

Catalog No. RP99347
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100 μL
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Catalog No. Quantity
RP99347 100 μL
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Catalog No. RP99347 Supplier Invitrogen™ Supplier No. RP99347

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63808 (PA5-63808. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Specifications

Accession Number Q9H598
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 140679
Name Human VGAT (aa 166-207) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias GABA and glycine transporter; GVAT; hVIAAT; mVGAT; mVIAAT; R75019; rGVAT; SLC32A1; solute carrier family 32 (GABA vesicular transporter), member 1; solute carrier family 32 member 1; vasicular inhibitory amino acid transporter 10 D; vesicular GABA transporter; Vesicular inhibitory amino acid transporter; Vgat; Viaat
Common Name VGAT
Gene Symbol SLC32A1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence LIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVN
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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