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Gibco™ Human TWEAK, Animal-Free Recombinant Protein, PeproTech®

Catalog No. AF31006250U
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250 μg
5 μg
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Catalog No. Quantity
AF31006250U 250 μg
AF310065UG 5 μg
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Catalog No. AF31006250U Supplier Gibco™ Supplier No. AF31006250UG

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Description

Recombinant Protein

Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Specifications

Accession Number O43508
For Use With (Application) Functional Assay
Format Lyophilized
Formulation protein with no preservative
Gene ID (Entrez) 8742
Molecular Weight (g/mol) 17 kDa
Name Human TWEAK, Animal-Free
Purification Method HPLC, SDS-PAGE
Quantity 250 μg
Endotoxin Concentration <0.1 EU/ μg
Gene Alias APO3 ligand; APO3/DR3 ligand; APO3L; Dr3l; Dr3lg; LOW QUALITY PROTEIN: tumor necrosis factor ligand superfamily member 12; MGC129581; MGC20669; TNF superfamily member 12; TNF-related weak inducer of apoptosis; TNFSF12; TNLG4A; tumor necrosis factor (ligand) superfamily member 12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4 A; tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; tumor necrosis factor superfamily member 12; TWEAK; UNQ181/PRO207; zgc:153941
Common Name TWEAK
Gene Symbol Tnfsf12
Biological Activity Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml
Product Type Protein
Conjugate Unconjugated
Recombinant Recombinant
Sequence MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH
Content And Storage -20°C
Activity Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/ml
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 98% by SDS-PAGE gel and HPLC analyses.
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