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  5. Invitrogen™ Human Trypsin 1/PRSS1 ELISA Kit

Invitrogen™ Human Trypsin 1/PRSS1 ELISA Kit

Catalog No. EH468RBX5
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EH468RBX5 5 x 96 Tests
EH468RB 96 Tests
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Description

ELISA

Human Trypsin 1/PRSS1 quantitates human Trypsin 1/PRSS1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Trypsin 1/PRSS1.

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.

Specifications

Accession Number Alpha-trypsin chain 1; Alpha-trypsin chain 2; anionic trypsin I; Anionic trypsin-1; beta-trypsin; Cationic trypsinogen; digestive zymogen; MGC120175; MGC149362; nonfunctional trypsin 1; pancreatic trypsin 1; pretrypsinogen I; Protease Serine 1; protease, serine 1; protease, serine 1 (trypsin 1); protease, serine, 1; protease, serine, 1 (trypsin 1); protease, serine, 2; PRSS1; PTRYI; RATPTRYI; Serine protease 1; TCR V beta 4.1; TRP1; TRY1; Try-1; TRY4; Trygn16; TRYP1; TRYP8; trypsin 1; Trypsin I; Trypsin-1; trypsinogen; trypsinogen 1; trypsinogen 16; trypsinogen A
Assay Range 0.29 to 200 ng/mL
Assay Sensitivity 0.29 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5644
Gene Symbol PRSS1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Trypsin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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