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  5. Invitrogen™ Human Tripeptidyl-peptidase I/TPP1 ELISA Kit

Invitrogen™ Human Tripeptidyl-peptidase I/TPP1 ELISA Kit

Catalog No. EH465RBX5
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5 x 96 Tests
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Description

ELISA

Human Tripeptidyl-peptidase I/TPP1 quantitates human Tripeptidyl-peptidase I/TPP1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Tripeptidyl-peptidase I/TPP1.

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Specifications

Accession Number Cell growth-inhibiting gene 1 protein; ceroid-lipofuscinosis, neuronal 2; CLN 2; Cln2; GIG 1; GIG1; growth-inhibiting protein 1; LPIC; lysosomal pepstatin insensitive protease; Lysosomal pepstatin-insensitive protease; MGC21297; SCAR7; TPP 1; TPP I; TPP1; TPP-1; TPPI; TPP-I; tripeptidyl aminopeptidase; tripeptidyl peptidase 1; tripeptidyl peptidase I; tripeptidyl peptidase-I; tripeptidyl-peptidase 1; Tripeptidyl-peptidase I; UNQ267/PRO304
Assay Range 10 to 2,500 pg/mL
Assay Sensitivity 10 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1200
Gene Symbol TPP1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target CLN2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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