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Invitrogen™ Human TRACP/PAP/ACP5 ELISA Kit

Catalog No. EH461RBX5
Encompass
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5 x 96 Tests
96 Tests
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EH461RBX5 5 x 96 Tests
EH461RB 96 Tests
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Catalog No. EH461RBX5 Supplier Invitrogen™ Supplier No. EH461RBX5

ELISA

Human TRACP/PAP/ACP5 quantitates human TRACP/PAP/ACP5 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human TRACP/PAP/ACP5.

Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen and liver. Its expression is increased in spleen and monocytes of patients with Gaucher's disease; spleenocytes and circulating leukocytes of patients with hairy cell leukemia; spleen of patients with Hodgkin's lymphoma and in the sera of patients undergoing active bone turnover.

Specifications

Accession Number acid phosphatase 5, tartrate resistant; acid phosphatase type V; ACP5; HPAP; human purple acid phosphatase; T5ap; Tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5 A; tartrate-resistant acid phosphatase 5 b; tartrate-resistant acid phosphatase type 5; TRACP; TRACP5a; TRACP5b; Trap; TR-AP; TRAPase; TrATPase; TTRRAP; type 5 acid phosphatase
Assay Range 0.05 to 10 ng/mL
Assay Sensitivity 0.05 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 54
Gene Symbol ACP5
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Tartrate Resistant Acid Phosphatase
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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