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Gibco™ Human TPO (Thrombopoietin) Recombinant Protein

Catalog No. phc9514
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10 μg
100 μg
1 mg
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Catalog No. Quantity
PHC9514 10 μg
PHC9511 100 μg
PHC9513 1 mg
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Catalog No. PHC9514 Supplier Gibco™ Supplier No. PHC9514
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Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Thrombopoietin in PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store this lyophilized preparation at <-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at <-20°C. Avoid repeated freeze/thaw cycles.

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

Specifications

Accession Number P40225
For Use With (Application) Bioactivity
Formulation PBS with no preservative
Gene ID (Entrez) 7066
Molecular Weight (g/mol) 85 kDa
Name Human TPO (Thrombopoietin)
Purification Method Purified
Quantity 10 μg
Storage Requirements -20°C
Regulatory Status RUO
Endotoxin Concentration <1 EU/μg
Gene Alias C-mpl ligand; Megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; Mgdf; MKCSF; ML; MPL ligand; Mpllg; MSA; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; TDH2A; THCYT1; THPO; Thrombopoietin; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; Tpo; TPX
Common Name Thrombopoietin
Gene Symbol THPO
Biological Activity ED50 = 0.5 - 5 ng/mL; determined by dose-dependent proliferation of human TF-1 cells.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage -20°C
Classification Carrier-Free
Activity ED50 = 0.5 - 5 ng/mL; determined by dose-dependent proliferation of human TF-1 cells.
Bioassay Proliferation of TF1 cells
Endotoxin Level <1 EU/ml
Shipping Condition Dry Ice
Expression System HEK293 cells
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype TPO
Research Category Endocrinology, Stem Cell Research, Angiogenesis
Product Line Gibco
Purity or Quality Grade >90% by SDS-PAGE
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