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Invitrogen™ Human TNF-alpha Recombinant Protein 
Recombinant Protein
Supplier: Invitrogen™ A42551
Description
Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.Specifications
| P01375 | |
| PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4 | |
| 17.4 kDa | |
| 7.4 | |
| 5 μg | |
| -20°C or -80°C if preferred | |
| APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor | |
| Tnf | |
| Protein | |
| Human | |
| Untagged | |
| Includes 1 vial of lyophilized protein. Store at -20 °C. | |
| E. coli | |
| Full Length, Recombinant | |
| TNF (Tumor Necrosis Factor) | |
| ≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC. |
| Control, Bioactivity, Immunohistochemistry, Surface Plasmon Resonance, Western Blot | |
| 7124 | |
| Human TNF-alpha | |
| Purified | |
| E. coli | |
| RUO | |
| TNF alpha | |
| 1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL 2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can Human TNF-alpha Recombinant Protein with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200)(Routinely tested). | |
| Unconjugated | |
| Recombinant | |
| Human TNF-alpha, amino acids Val77-Leu233 (Accession # NP_000585.2) with an N-terminal Met | |
| 1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.<br><br>2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can Human TNF-alpha Recombinant Protein with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200)(Routinely tested). | |
| Cytokines & Receptors | |
| Lyophilized | |
| Immunology, Inflammation, Oncology, Signal Transduction | |
| tumor necrosis factor |