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  5. Invitrogen™ Human SP-D ELISA Kit

Invitrogen™ Human SP-D ELISA Kit

Catalog No. EH436RBX5
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5 x 96 Tests
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Description

ELISA

Human SP-D quantitates human SP-D in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human SP-D.

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages.

Specifications

Accession Number AI573415; COLEC7; Collectin7; Collectin-7; CP4; EGK
Assay Range 0.12 to 30 ng/mL
Assay Sensitivity 0.12 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6441
Gene Symbol SFTPD
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target SFTPD
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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