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Invitrogen™ Human L-Selectin (CD62L) (Soluble) ELISA Kit

Catalog No. p-7092004
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10 x 96 Tests
96 Tests
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Catalog No. Quantity
5017974 10 x 96 Tests
5017967 96 Tests
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Catalog No. 5017974 Supplier Invitrogen™ Supplier No. BMS206TEN
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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sL-selectin
HRP-Conjugate anti-human sL-selectin monoclonal antibody, ready to use
Human sL-selectin Standard lyophilized, 50ng/mL upon reconstitution
Sample Diluent
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

ELISA

The Human L-Selectin (CD62L) ELISA quantitates Hu L-Selectin in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu L-Selectin. Principle of the method The Human L-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

Specifications

Accession Number P14151
Assay Range 0.4 to 25.0 ng/mL
Assay Sensitivity 0.198 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6402
Gene Symbol CD62L,LAM1,LECAM1,LEU8,LNHR,LSEL,LYAM1,PLNHR,TQ1
Interassay CV 4.2%
Intraassay CV 3.7
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Storage Requirements 2°C to 8°C
Synonym Lymphocyte adhesion molecule 1, CD62L, Leu-8
Target L-Selectin (CD62L)
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 10 mins
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