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  5. Invitrogen™ Human Serpin A3/Alpha-1-Antichymotrypsin ELISA Kit

Invitrogen™ Human Serpin A3/Alpha-1-Antichymotrypsin ELISA Kit

Catalog No. EH411RBX5
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5 x 96 Tests
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EH411RBX5 5 x 96 Tests
EH411RB 96 Tests
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Description

ELISA

Human Serpin A3/Alpha-1-Antichymotrypsin quantitates human Serpin A3/Alpha-1-Antichymotrypsin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Serpin A3/Alpha-1-Antichymotrypsin.

SERPINA encodes Alpha 1-ACT. It is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain. Regulation of the serine proteases and serine protease inhibitors plays an important role in neuromuscular differentiation. Prostate specific antigen (PSA), a chymotrypsin-like serine protease, is predominantly complexed to Alpha-1-ACT.

Specifications

Accession Number AACT; ACT; Alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin His-Pro-less; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; growth-inhibiting protein 24; growth-inhibiting protein 25; MGC88254; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin family A member 3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; SERPINA3
Assay Range 0.137 to 100 ng/mL
Assay Sensitivity 0.14 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 12
Gene Symbol SERPINA3
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target SERPINA3
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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