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Invitrogen™ Human SARS-CoV-2 N ELISA Kit

Catalog No. EH490RB
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96 Tests
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EH490RB 96 Tests
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Catalog No. EH490RB Supplier Invitrogen™ Supplier No. EH490RB
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Description

ELISA

The Human SARS-CoV-2 Nucleocapsid ELISA quantitates Nucleocapsid in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Human Nucleocapsid. Principle of the method The Human SARS-CoV-2 Nucleocapsid solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). The nucleocapsid protein (N) was one of the structural proteins of SARS-CoV (N, S, sE, M). The severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein is the most abundant protein in the virus-infected cells. The SARS-CoV-2 S gene has 76% amino acid similarity to the SARS-CoV S gene, and nonsynonymous mutations developed in the S protein as the SARS-CoV epidemic developed, reports have shown that the crystal structure of the SARS-CoV-2 nucleocapsid protein is similar to those of previously described coronavirus N proteins, but their surface electrostatic potential characteristics are distinct.

Specifications

Assay Range 0.069-50 ng/mL
Assay Sensitivity 0.069 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Nasopharyngeal Swab, Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Coronavirus; COVID; COVID 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Nucleocapsid; SARS-CoV2; SARS-CoV-2Coronavirus; COVID; COVID 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Nucleocapsid; SARS-CoV2; SARS-CoV-2;
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 25 μL, Serum 25 μL, Supernatant 25 μL
Shipping Condition Wet Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target SARS Coronavirus Nucleocapsid
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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