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Invitrogen™ Human Ribophorin I (aa 35-171) Control Fragment Recombinant Protein

Catalog No. RP104924
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Catalog No. Quantity
RP104924 100 μL
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Catalog No. RP104924 Supplier Invitrogen™ Supplier No. RP104924

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (93%), Rat (93%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65662 (PA5-65662. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.

Specifications

Accession Number P04843
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 6184
Name Human Ribophorin I (aa 35-171) Control Fragment
Quantity 100 μL
Gene Alias AU018702; D6Wsu137e; Dolichyl-diphosphooligosaccharide--protein; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; oligosaccharyltransfe; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; QccE-21639; QtsA-18349; RBPH1; RIBI; ribophorin I; Ribophorin-1; RPN1; Rpn-1; RPN-I; unnamed protein product
Common Name Ribophorin I
Gene Symbol RPN1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence DVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQ
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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