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Invitrogen™ Human Profilin 1 ELISA Kit

Catalog No. EEL187
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96 Tests
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Catalog No. Quantity
EEL187 96 Tests
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Catalog No. EEL187 Supplier Invitrogen™ Supplier No. EEL187

Description

ELISA

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Specifications

Accession Number P07737
Assay Range 78.13 to 5000 pg/mL
Assay Sensitivity 46.88 pg/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5216
Gene Symbol Pfn1
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Profilin 1
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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