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  5. Invitrogen™ Human PD-ECGF ELISA Kit

Invitrogen™ Human PD-ECGF ELISA Kit

Catalog No. EH364RBX5
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5 x 96 Tests
96 Tests
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EH364RBX5 5 x 96 Tests
EH364RB 96 Tests
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Description

ELISA

Human PD-ECGF quantitates human PD-ECGF in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PD-ECGF.

A platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.

Specifications

Accession Number P19971
Assay Range 0.4-100 ng/mL
Assay Sensitivity 0.4 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 2900072D10Rik; ECGF; Ecgf1; endothelial cell growth factor 1 (platelet-derived); gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; OTTHUMP00000196767; OTTHUMP00000196770; PD TP; PDECGF; PD-ECGF; Pdgfec; platelet derived growth factor, endothelial cell; Platelet-derived endothelial cell growth factor; TdRPase; thymidine phosphorylase; TP; TYMP2900072D10Rik; ECGF; Ecgf1; endothelial cell growth factor 1 (platelet-derived); gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; OTTHUMP00000196767; OTTHUMP00000196770; PD TP; PDECGF; PD-ECGF; Pdgfec; platelet derived growth factor, endothelial cell; Platelet-derived endothelial cell growth factor; TdRPase; thymidine phosphorylase; TP; TYMP;
Gene ID (Entrez) 1890
Gene Symbol TYMP
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target Thymidine Phosphorylase
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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