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Invitrogen™ Human PARP1 His-tag Recombinant Protein

Catalog No. A42573
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5 μg
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Catalog No. Quantity
A42573 5 μg
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Catalog No. A42573 Supplier Invitrogen™ Supplier No. A42573
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Description

Recombinant Protein

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

Specifications

Accession Number P09874
For Use With (Application) Control, Bioactivity, Functional Assay, Immunohistochemistry, Western Blot
Formulation 20mM tris with 2mM EDTA, 10% glycerol, 300mM NaCl, 0.5mM TCEP and no preservative; pH 7.5
Gene ID (Entrez) 142
Molecular Weight (g/mol) 114.5 kDa
Name Human PARP1 His-tag
pH Range 7.5
Purification Method Purified
Quantity 5 μg
Source Insect cells
Storage Requirements -20°C or -80°C if preferred
Regulatory Status RUO
Endotoxin Concentration <1.0 EU/μg
Gene Alias 5830444G22Rik; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); Adprp; Adprt; ADPRT 1; Adprt1; AI893648; ARTD1; C80510; DNA ADP-ribosyltransferase PARP1; I79_014850; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; pADPRT-1; PARP; PARP1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); poly [ADP-ribose] polymerase 1; poly ADP-ribose polymerase; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1; sPARP-1; synthetase fragment (AA 67); Unknown (protein for IMAGE:8023736); unnamed protein product
Common Name PARP1
Gene Symbol Parp1
Biological Activity ED50 = 0.2 - 1.0 μg/mL; determined by the binding of immobilized human PARP His-tag (2 μg/mL) to biotinylated human HSP70 His-tag in a functional ELISA.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-tag
Sequence Human PARP1, amino acids Met1-Trp1014 ( Accession # NP_001609.2) with a C-terminal His-tag
Content And Storage Store at -80 °C.
Activity ED50 = 0.2 - 1.0 μg/mL; determined by the binding of immobilized human PARP His-tag (2 μg/mL) to biotinylated human HSP70 His-tag in a functional ELISA.
Endotoxin Level < 1 EU/μg
Expression System Insect cells
Form Liquid
Protein Subtype Other Proteins
Research Category DNA Replication & Repair
Purity or Quality Grade >90% by SDS-PAGE
Protein poly (ADP-ribose) polymerase 1
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