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Invitrogen™ Human Parathyroid Hormone Related Protein ELISA Kit

Catalog No. EEL177
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96 Tests
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EEL177 96 Tests
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Catalog No. EEL177 Supplier Invitrogen™ Supplier No. EEL177

Description

ELISA

The Human Parathyroid Hormone Related Protein (PTHrP) ELISA quantitates PTHrP in serum, plasma and other biological fluids. Principle of the method The Human Parathyroid Hormone Related Protein (PTHrP) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

The protein encoded by this gene is a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, in-frame and downstream of the initiator AUG codon, to give rise to nuclear forms of this hormone.

Specifications

Accession Number P12272
Assay Range 15.63-1000 pg/mL
Assay Sensitivity 9.38 pg/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias BDE2; calciotropic hormone, growth factor; HHM; osteostatin; parathyroid hormone like hormone; parathyroid hormone related peptide; parathyroid hormone-like hormone; parathyroid hormone-like hormone preproprotein; parathyroid hormone-like peptide; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-related peptide; parathyroid hormone-related protein; parathyroid-like peptide; PLP; PTHLH; PTH-like; PTHR; PTH-related peptide; PTH-related protein; Pthrp; PTH-rP; PTHrP[107-139]; PTHrP[1-36]; PTHrP[38-94]BDE2; calciotropic hormone, growth factor; HHM; osteostatin; parathyroid hormone like hormone; parathyroid hormone related peptide; parathyroid hormone-like hormone; parathyroid hormone-like hormone preproprotein; parathyroid hormone-like peptide; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-related peptide; parathyroid hormone-related protein; parathyroid-like peptide; PLP; PTHLH; PTH-like; PTHR; PTH-related peptide; PTH-related protein; Pthrp; PTH-rP; PTHrP[107-139]; PTHrP[1-36]; PTHrP[38-94];
Gene ID (Entrez) 5744
Gene Symbol PTHLH
Interassay CV <10%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Refer to product documentation for component specific storage temperature.
Target PTHLH
Test Time 1 hr 20 min
Total Assay Time 3 hr 30 min
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