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Invitrogen™ Human NR5A1 (aa 223-272) Control Fragment Recombinant Protein
Description
Highest antigen sequence indentity to the following orthologs: Mouse (88%), Rat (88%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-139965. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system. SF-1 has been shown to be essential for fetal development of the hypothalamus, pituitary gland, gonads, and the adrenal gland. SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
Specifications
Specifications
| Accession Number | Q13285 |
| Concentration | ≥5.0 mg/mL |
| For Use With (Application) | Neutralization, Control |
| Formulation | PBS, 1M urea with no preservative; pH 7.4 |
| Gene ID (Entrez) | 2516 |
| Name | Human NR5A1 (aa 223-272) Control Fragment |
| pH Range | 7.4 |
| Purification Method | Purified |
| Quantity | 100 μL |
| Storage Requirements | -20°C, Avoid Freeze/Thaw Cycles |
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