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PeproTech Human NOV ELISA Development Kit (ABTS), PeproTech®

Catalog No. 900K338K
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900K338K 10 x 96 Tests
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Catalog No. 900K338K Supplier PeproTech Supplier No. 900K338K

Description

ELISA

900-K338K consists of: 900-K338 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate). Human NOV ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human NOV in a sandwich ELISA format. Human NOV ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells. The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm's tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities.

Specifications

Accession Number P48745
Assay Range Lot-specific
Assay Sensitivity Lot-specific
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Growth Factors & Receptors
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias C130088N23Rik; CCN family member 3; CCN3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; IGFBP9; IGFBP-9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; nephroblastoma overexpressed gene; nephroblastoma overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; NOV; NovH; protein NOV homologC130088N23Rik; CCN family member 3; CCN3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; IGFBP9; IGFBP-9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; nephroblastoma overexpressed gene; nephroblastoma overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; NOV; NovH; protein NOV homolog;
Gene ID (Entrez) 4856
Gene Symbol CCN3
Kit Contents This Human NOV ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately., Detection Antibody, Protein Standard, HRP-conjugate
Label or Dye Biotin
Quantity 10 x 96 Tests
Regulatory Status RUO
Shipping Condition Room temperature
Storage Requirements Refer to product documentation for component specific storage temperature.
Target NOV
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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