Gibco™ Human Noggin Recombinant Protein 

Description

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 10 mM acetic acid to a concentration of 0.1-1.0 mg/mL. Apportion the reconstituted protein into working aliquots and store at =-20°C. Make any further dilutions of the reconstituted protein in appropriate buffered solution containing a carrier protein, such as 0.1% HSA or BSA. Storage: The lyophilized protein is stable at room temperature for 3 weeks. Store at -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at 4°C for 2-7 days, or at -20°C. Avoid repeated freeze-thaw cycles.

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.