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Invitrogen™ Human NACAD (aa 477-569) Control Fragment Recombinant Protein
Description
Highest antigen sequence indentity to the following orthologs: Mouse (46%), Rat (46%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-54294. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
May prevent inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). May bind to nascent polypeptide chains as they emerge from the ribosome and block their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. May also reduce the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).
Specifications
Specifications
| Accession Number | O15069 |
| Concentration | ≥5.0 mg/mL |
| For Use With (Application) | Neutralization, Control |
| Formulation | PBS, 1M urea with no preservative; pH 7.4 |
| Gene ID (Entrez) | 23148 |
| Name | Human NACAD (aa 477-569) Control Fragment |
| pH Range | 7.4 |
| Purification Method | Purified |
| Quantity | 100 μL |
| Storage Requirements | -20°C, Avoid Freeze/Thaw Cycles |
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