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  5. Invitrogen™ Human MEPE/OF45 ELISA Kit

Invitrogen™ Human MEPE/OF45 ELISA Kit

Catalog No. EH320RBX5
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5 x 96 Tests
96 Tests
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EH320RBX5 5 x 96 Tests
EH320RB 96 Tests
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Description

ELISA

Human MEPE/OF45 quantitates human MEPE/OF45 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MEPE/OF45.

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. MEPE promotes renal phosphate excretion and modulates mineralization. It can be phosphorylated by secreted protein kinases. MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines. It is a 525-amino acid protein with a calculated molecular weight of 58 kDa.

Specifications

Accession Number matrix extracellular phosphoglycoprotein; matrix extracellular phosphoglycoprotein with ASARM motif (bone); matrix, extracellular phosphoglycoprotein with ASARM motif (bone); Mepe; OF45; Osteoblast/osteocyte factor 45; Osteoregulin
Assay Range 26 to 2,000 ng/mL
Assay Sensitivity 8.4 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 56955
Gene Symbol MEPE
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target MEPE
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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