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Gibco™ Human Melanocyte Growth Supplement (HMGS) DFS Item

Human Melanocyte Growth Supplement (HMGS)

Supplier:  Gibco™ S0025

Catalog No. S0025


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Description

Description

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5 ml bottle of HMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of basal Medium 254 or Medium 254CF.

When a 500 ml bottle of medium is supplemented with HMGS, the final concentrations of the components in the supplemented medium are:

  • Bovine pituitary extract (BPE) (0.2% v/v)
  • Fetal bovine serum (0.5% v/v)
  • Recombinant human insulin-like growth factor-I (1μg/ml)
  • Bovine transferrin (5μg/ml)
  • Basic fibroblast growth factor (3 ng/ml)
  • Hydrocortisone (0.18μg/ml)
  • Heparin (3μg/ml)
  • Phorbol 12-myristate 13-acetate (PMA) (10 ng/ml)
    • Specifications

    Specifications

    Store in freezer (-5 to -20°C).
    100 X
    Growth Supplement
    Cascade Biologics
    Dry Ice
    Human
    Mammalian Cells
    Liquid
    Sterile
    5 mL
    Human
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    For Research Use Only. Not for use in diagnostic procedures.