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  5. Invitrogen™ Human JAM-C ELISA Kit

Invitrogen™ Human JAM-C ELISA Kit

Catalog No. EH285RBX5
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5 x 96 Tests
96 Tests
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EH285RBX5 5 x 96 Tests
EH285RB 96 Tests
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Description

ELISA

Human JAM-C quantitates human JAM-C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human JAM-C.

JAM3 is involved in tight junctions and represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.

Specifications

Accession Number Q9BX67
Assay Range 0.12-30 ng/mL
Assay Sensitivity 0.12 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 1110002N23Rik; JAM-2; JAM3; JAM-3; JAMC; JAM-C; Jcam3; junction adhesion molecule 3; junction cell adhesion molecule 3; junction cell adhesion molecule C; junctional adhesion molecule 3; Junctional adhesion molecule C; sJAM-C; Soluble form of JAM-C; UNQ859/PRO18681110002N23Rik; JAM-2; JAM3; JAM-3; JAMC; JAM-C; Jcam3; junction adhesion molecule 3; junction cell adhesion molecule 3; junction cell adhesion molecule C; junctional adhesion molecule 3; Junctional adhesion molecule C; sJAM-C; Soluble form of JAM-C; UNQ859/PRO1868;
Gene ID (Entrez) 83700
Gene Symbol JAM3
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target JAM3
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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